Yeast Tutorial

This tutorial is a step-by-step guide to reproduce the results of the paper.

Downloading the data

The dataset used in the paper is available at figshare:

Shen, Xing-Xing (2018). Tempo and mode of genome evolution in the budding yeast subphylum. figshare. Dataset. https://doi.org/10.6084/m9.figshare.5854692.v1

The data can be downloaded and extracted with these commands:

wget https://ndownloader.figshare.com/files/13092791 -O 0_332yeast_genomes.zip
unzip 0_332yeast_genomes.zip
cd 0_332yeast_genomes
unzip 332_genome_annotations.zip
cd pep

Setup

You can create the input file for the 24 species shown in the paper but saving this text as yeast24.csv:

file,data_type
ambrosiozyma_kashinagacola.max.pep,Protein
ambrosiozyma_monospora.max.pep,Protein
ascoidea_asiatica.max.pep,Protein
ascoidea_rubescens.max.pep,Protein
candida_auris.max.pep,Protein
clavispora_lusitaniae.max.pep,Protein
cyberlindnera_jadinii.max.pep,Protein
cyberlindnera_fabianii_JCM3601.max.pep,Protein
eremothecium_cymbalariae.max.pep,Protein
eremothecium_gossypii.max.pep,Protein
hanseniaspora_uvarum.max.pep,Protein
hanseniaspora_valbyensis.max.pep,Protein
kluyveromyces_lactis.max.pep,Protein
kluyveromyces_marxianus.max.pep,Protein
lachancea_nothofagi.max.pep,Protein
lachancea_quebecensis.max.pep,Protein
metschnikowia_hibisci.max.pep,Protein
metschnikowia_shivogae.max.pep,Protein
ogataea_methanolica.max.pep,Protein
ogataea_parapolymorpha.max.pep,Protein
ogataea_polymorpha.max.pep,Protein
saccharomycopsis_malanga.max.pep,Protein
wickerhamomyces_anomalus.max.pep,Protein
wickerhamomyces_ciferrii.max.pep,Protein

Running Orthoflow

The following command will run Orthoflow:

orthoflow --files yeast24.csv --config infer_tree_with_cds_seqs=False

Note

We need to turn off the option infer_tree_with_cds_seqs because we are using protein sequences.

Results

The results will be saved in the results folder.

For example, you should see a file called results/supermatrix/supermatrix_tree_render.protein.png

../_images/yeast24_supermatrix_tree_render.protein.png

Mini Test

The repository includes a mini version of the dataset that can be used for testing. It includes a handful of genes from these files:

  • ambrosiozyma_kashinagacola.max.pep

  • ambrosiozyma_monospora.max.pep

  • ascoidea_asiatica.max.pep

  • ascoidea_rubescens.max.pep

  • candida_auris.max.pep

To run this mini test, do the following from the root of the repository:

cd tests/test-data-yeast/
orthoflow --files yeast5.csv --config infer_tree_with_cds_seqs=False

This test is run as part of the continuous integration process. See the .github/workflows/yeast.yml file for details.

The current status of this test is shown here: yeast badge